Fig. 4 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 4. Ultrametric tree constructed using Bayesian inference and based on 29 haplotype sequences (658bp) of the cytochrome oxidase c subunit I gene of specimens currently identified as Lasioglossum villosulum (Kirby, 1802), Lasioglossum medinai (Vachal, 1895) and one as Lasioglossum berberum (Benoist, 1941). Each label corresponds to one roman letter which encompasses all sequence from a haplotype (for more details see Table 3). A. Lasioglossum medinai; B–D: three supported clusters (a fourth cluster could be defined in C) within Lasioglossum villosulum. This phylogenetic tree is rooted using Lasioglossum bluethgeni Ebmer, 1971 as outgroup (label III, voucher AP222). Posterior probabilities are given at nodes. The three colour gradients on the tree correspond to morphological delineation. Results of the species delimitations analyses are represented on the right side of the figure: the Bayesian Poisson Tree Process (bPTP) analyses based on the trees obtained using Bayesian inference (BI) or maximum likelihood (ML); The Generalized Mixed Yule Coalescent (GMYC) analysis resulting in seven candidate species (using the single threshold represented as a red line on the tree) and five alternative scenarios. Numbers at the top of the columns corresponds to the number of candidate species in the GMYC analysis.